Part:BBa_K4212037:Design
ChiS4
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1915
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1915
Illegal NheI site found at 1011 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1915
Illegal BamHI site found at 1900 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1915
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1915
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part consists the fusion protein consisted of CotG and ChiS, in which we combined these parts into level 1 Golden Gate Construct. Our dry lab team predicted the functional model of this chimera and our wet lab intended to test the effectiveness of this model. Promoter hyper-spank is an inducible promoter, which was designed for high-level protein expression in B.subtilis and it was previously used by other iGEM teams. We added a flexible linker to this construct to test if it works better than the construct without the linker. We tried out different linkers to compare their effectiveness.Promoter hyper-spank is an inducible promoter, which was designed for high-level protein expression in B.subtilis and it was previously used by other iGEM teams. tL3S2P21 is a strong terminator, which helps to increase the production of desired protein. The addition of Isopropyl β-D-1-thiogalactopyranoside (IPTG) can induce gene expression under the control of the promoter hyperspank. tL3S2P21 is a strong terminator, which helps to increase the production of desired protein.
Source
Synthetic construct.